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Methodology

The basis of the Winkler procedure is that the oxygen in a sea water sample is made to oxidize iodide ion to iodine quantitatively (in the presence of an alkaline solution of manganese (II) ion); and the amount of iodine generated in this fashion is determined by titration with a standard thiosulfate solution. The end-point is located using starch as a visual indicator for the presence of iodine. The amount of oxygen present in the original sample can then be computed from the titrator.

Reference

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